The other passengers were transported by ground to three area hospitals.
One player, Nicole Rossi, of Collegeville, was treated at Carlisle Regional Medical Center and released Saturday afternoon, a spokeswoman said.
The crash happened around 9 a.m. Saturday at mile post 227, about a mile east of the Carlisle exit, said Bill Capone, spokesman for the Pennsylvania Turnpike Commission.
The bus was heading eastbound from Greensburg to Lancaster, Capone said, taking 23 players and 3 coaches from the Seton Hill University women's lacrosse team to a game at Millersville University.
The Seton Hill University community is deeply saddened," Seton Hill spokeswoman Kary L. Coleman said in a release.
Quigley, who is survived by her husband, Glenn Quigley, and young son, Gavin, had just begun her second season with the Seton Hill women's lacrosse program, according to Coleman. She led the Griffins to 11 victories in her first season.
A Baltimore, Md. native, Quigley came to Seton Hill from Erskine College in Due West, S.C., where she started the NCAA Division II program.
Prior to that, she worked as the assistant lacrosse coach at Duquesne University, her alma mater. She graduated from Duquesne with a major in secondary education and minors in math and English.
The university will offer a memorial Mass for Quigley and pray for Guaetta and all those injured, Sunday at 7 p.m. in St. Joseph Chapel on the university's campus in Greenburg, southeast of Pittsburgh.
The cause of the accident is still under investigation, Capone said, but the crash did not appear to involve any other cars. The bus went over a guiderail, through a fence, and struck a tree several dozen yards from the road, he said.
The fence is used to denote property lines, Capone said, and not designed to stop a vehicle. The guiderail cannot always stop cars either:
"The speed and angle that this bus was going, maybe under other conditions it might deflect it and maybe grab it and keep it from going through," Capone said, "But in this case it either jumped it or went over it in some fashion."
Contact Jonathan Lai at 856-779-3220, email@example.com, or on Twitter @elaijuh.
This story includes contributions The Associated Press.